AUTOMATIC MICRO-ELISA SYSTEM AND APPLICATION TO RAPID hsCRP DIAGNOSIS
نویسندگان
چکیده
We developed a fully-automated micro ELISA (Enzyme-Linked ImmunoSorbent Assay) system utilizing beads-based immunoassay format. The ELISA system was applied to hsCRP (high sensitivity C-reactive protein) detection for point-of-care (POC) diagnosis of cardiac infarction or diabetes mellitus. The performance was evaluated with human serum sample, and rapid (8 min), sensitive (50-5000 ng/mL), high precision (3%) and easy handling for general practitioners (fully-automated) was demonstrated. Our system becomes powerful and practical diagnostic tool for POC diagnosis.
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